RNase A Solution from Promega

Product Specs
ItemRNase A Solution
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA7973
Quantity1ml
Concentration4mg/ml

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoaScientific ReportsNovember 3, 2023Marina El Kamouh, Aurélien Brionne, Amin Sayyari, Audrey Laurent, Catherine Labbé15 min at 12,000g at 4 °C. The new DNA pellet was left to air dry. It was then resuspended in RNAse (A7973; Promega) 100 µg/mL final concentration in nuclease-free water and incubated for 1 h at 37 °C. DNA concentration

(2) IRAK3 Knockout and Wildtype THP-1 Monocytes as Models for Endotoxin Detection Assays and Fusobacterium nucleatum Bacteriophage FNU1 Cytokine InductionInternational Journal of Molecular SciencesOctober 12, 2023Siti Saleha Binte Mohamed Yakob Adil, Mwila Kabwe, Cassandra Cianciarulo, Trang Hong Nguyen, Helen Irving, Joseph Tuccichloride (MgCl2), 1 mL of DNase I (Promega, M6101, Sydney, NSW, Australia) and 1 mL RNase A (Promega, A7973, Australia) were added to 1 mL of bacteriophage suspension. After incubation at room temperature for

(3) Single-cell profiling of transcriptome and histone modifications with EpiDamIDMolecular cellMay 19, 2022Rang FJ, de Luca KL, de Vries SS, Valdes-Quezada C, Boele E, Nguyen PD, Guerreiro I, Sato Y, Kimura H, Bakkers J, et al.37%,Sigma,Cat#F8775-500ml; CAS: 50-00-0 Glycine,Sigma,Cat#50046-250 g; CAS: 56-40-6 RNase A,Promega,Cat#A7973 Proteinase K,Roche,Cat#3115879001; CAS: 39450-01-6 Protein G beads,Thermo Fisher Scientific,Cat#88847 Bovine Serum Albumin

(4) Exosome-Encased Nucleic Acid Scaffold Chemotherapeutic Agents for Superior Anti-Tumor and Anti-Angiogenesis ActivityACS Bio & Med Chem AuApril 20, 2022Ryan P. McNamara, Anthony B. Eason, Yijun Zhou, Rachele Bigi, Jack D. Griffith, Lindsey M. Costantini, Michelle A. Rudek, Nicole M. Anders, Blossom A. Damania, Dirk P. DittmerPBS. Non-EV-encased nucleic acid complexes were digested with the addition of 50 μg/mL RNaseA (Promega A7973) for 30 min at 37 °C. A full, extended protocol can be found in ref (15). Fractionations and Drug-Loaded

(5) Class III Peroxidases in Response to Multiple Abiotic Stresses in Arabidopsis thaliana Pyrenean PopulationsInternational Journal of Molecular SciencesApril 2, 2022Ali Eljebbawi, Bruno Savelli, Cyril Libourel, José Manuel Estevez, Christophe DunandSouffelweyersheim, France), 1% PVP, 1% sodium metabisulfite (S9000, Sigma-Aldrich), 10 μg/mL RNase A (A7973, Promega, Charbonnières-les-Bains, France), and 0.5 mg/mL proteinase K (V3021, Promega)) at 65 °C for

(6) Self-capping of nucleoprotein filaments protects the Newcastle disease virus genomeeLifeJuly 10, 2019Xiyong Song et al.Sciences" "Chemicalcompound, drug",elastase,SIGMA,E8140-1UN, "Chemicalcompound, drug",RNase A,Promega,A7973, "Software, algorithm",RELION 1.4,PMID: 23000701,,https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page "Software,

(7) The Carboxyl Terminus of Tegument Protein pUL21 Contributes to Pseudorabies Virus NeuroinvasionJournal of virologyApril 1, 2019Yan K, Liu J, Guan X, Yin YX, Peng H, Chen HC, Liu ZF.EGTA] with 10 mM DTT, protease inhibitor cocktail, 0.1 U/␮l DNase I, and 100 ␮g/ml RNase A (catalog no. A7973; Promega). Following incubations at 37°C for 30 min and 4°C overnight, the capsids were centrifuged

(8) Comparison of European eel sperm cryopreservation protocols with standardization as a targetAquacultureJanuary 1, 2019J.G. Herranz-Jusdado et al.After centrifugation, the dried DNA pellet was mixed with 100 µg/mL 224 RNase in water (Promega, A7973) and incubated 1 h at 37 °C. Whole DNA methylation level 225 was estimated using LUMA (luminometric

(9) A novel variant of torque teno virus 7 identified in patients with Kawasaki diseasePLOS ONEDecember 28, 2018James B. Thissen, Mariko Isshiki, Crystal Jaing, Yoshiro Nagao, Dayanara Lebron Aldea, Jonathan E. Allen, Masafumi Izui, Thomas R. Slezak, Takafumi Ishida, Tetsuya SanoGermany), 1 μl Baseline Zero DB0711K (Epicentre, Madison, WI), 1 μl AM2238 Turbo DNase (Ambion), and 1μl A7973 RNase A (Promega, Madison, WI), then incubated at 37°C for 90 minutes. Nucleases were inactivated by

(10) Large-scale, cross-flow based isolation of highly pure and endocytosis-competent extracellular vesiclesJournal of Extracellular VesiclesDecember 1, 2018Ryan P. McNamara, Carolina P. Caro-Vegas, Lindsey M. Costantini, Justin T. Landis, Jack D. Griffith, Blossom A. Damania, Dirk P. Dittmerpellet was resuspended in 250 μL of PBS, and the solution was then incubated with RNaseA (Promega, P: A7973) at a final concentration of 50 µg/mL at 37°C for 30 min. The solution was then diluted to 5 mL and

(11) Growth, stoichiometry and cell size; temperature and nutrient responses in haptophytesPeerJSeptember 5, 2017Lars Fredrik Skau, Tom Andersen, Jan-Erik Thrane, Dag Olav HessenGibco, Grand Island, NY, USA) was added. The other duplicates were then added 20 μL of 0.1% RNase A (A7973; Promega, Madison, WI, USA). Immediately after the RNase mixture was added, the well plate was heat

(12) Changes in Stoichiometry, Cellular RNA, and Alkaline Phosphatase Activity of Chlamydomonas in Response to Temperature and NutrientsFrontiers in MicrobiologyJanuary 23, 2017Dag O. Hessen, Ola T. Hafslund, Tom Andersen, Catharina Broch, Nita K. Shala, Marcin W. Wojewodzicof 20 μl RNase-free water (Gibco BRL1071), and in the other set of duplicates 20 μl of 0.1% RNase A (A7973, Promega). Immediately after the RNase mixture was added, the well plate was incubated at 37°C on a

(13) RNA regulatory networks diversified through curvature of the PUF protein scaffoldNature communicationsSeptember 14, 2015Wilinski D, Qiu C, Lapointe CP, Nevil M, Campbell ZT, Tanaka Hall TM, Wickens M.°C. A measure of 30 μl of RQ1 DNASE (Promega, M6101) and 10 μl 1:10,000 dilution of RNAse A (Promega A7973) were added and incubated at 37 °C for 10 min. The lysate was cleared by centrifugation then incubated

(14) A posttranscriptional mechanism that controls Ptbp1 abundance in the Xenopus epidermisMolecular and cellular biologyFebruary 1, 2015Méreau A, Anquetil V, Lerivray H, Viet J, Schirmer C, Audic Y, Legagneux V, Hardy S, Paillard L.irradiation (4,000 ␮J/cm2; Stratalinker). The extracts were treated with 2 ␮l RNase A (catalog number A7973; Promega) before immunoprecipitation as described above. Real-time and radioactive PCR. Real-time PCR

(15) Time resolved DNA occupancy dynamics during the respiratory oscillation uncover a global reset point in the yeast growth programMicrobial CellSeptember 1, 2014Cornelia Amariei et al.concentrations that are shown in parentheses. MNase (150 U) and RNase A (5 μL; Promega, Japan, catalogue number: A7973) were then added, and samples were incubated (37°C; 30 min). The NaCl, MgCl2, CaCl2 and MNase were omitted

(16) Absence of MGST1 mRNA and protein expression in human neuroblastoma cell lines and primary tissueFree Radical Biology and MedicineApril 1, 2014Michael J. Kelner, Mitchell B. Diccianni, Alice L. Yu, Mary R. Rutherford, Leita A. Estes, Ralf MorgensternGenomic DNA purification method (Promega Lysis Solution #A7943, Protein Precipitation Solution #A7953, and RNase Solution #A7973) and DNA content quantified by spectrophotometry. Genomic DNA (15 – 30 ug) was incubated with an excess

(17) Transcription factor Foxp3 and its protein partners form a complex regulatory networkNature immunologyOctober 1, 2012Rudra D, deRoos P, Chaudhry A, Niec RE, Arvey A, Samstein RM, Leslie C, Shaffer SA, Goodlett DR, Rudensky AY.(Invitrogen #18047–019; 20 Kunitz units/ml), and in some experiments with both DNase and RNase (Promega A7973; 10 µg/ml). The nuclear lysates were spun for 30 min at 15000g for 30 min and protein concentration

(18) Single-molecule and population probing of chromatin structure using DNA methyltransferasesMethodsMarch 1, 2007Jessica A KILGORE, Scott A HOOSE, Tanya L GUSTAFSON, Weston PORTER, Michael P KLADDE#A1120). 2. Promega nuclei lysis solution (cat. #A7941). 3. Promega RNase A solution, 4 mg/ml (cat. #A7973). 4. Promega protein precipitation solution (cat. #A7951). 5. Isopropanol. Caution: Highly flammable.

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RNase A Solution

RNase A Solution from Promega

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RNase A Solution from Promega

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